Table I.
Constructb | Cell Typea
|
|||||
---|---|---|---|---|---|---|
Cortex | Endodermis | Phloem
|
Xylem | Pith | ||
SE | Adjacent to SEc | |||||
pSLJ755I5 | − | − | − | − | − | − |
RTM1:RTM1 | − | − | − | − | − | − |
RTM1:GFP-RTM1 | − | − | + | + | − | − |
RTM2:RTM2-GFP | − | − | + | − | − | − |
35S:GFP | + | + | ndd | + | + | + |
35S:GFP-RTM1 | + | + | + | + | + | + |
+, Cell type contained GFP when examined by epifluorescence microscopy; −, no fluorescence detected in cell type.
DNA construct (see Fig. 2) expressed in transgenic Arabidopsis plants.
Phloem-associated cells other than SEs.
nd, Not determined. High levels of diffuse GFP in cells and tissues adjacent to SEs prevented determination of whether or not SEs contained GFP.