Table I.
Cell types containing GFP fluorescence in stem tissue of transgenic Arabidopsis plants
| Constructb | Cell Typea
|
|||||
|---|---|---|---|---|---|---|
| Cortex | Endodermis | Phloem
|
Xylem | Pith | ||
| SE | Adjacent to SEc | |||||
| pSLJ755I5 | − | − | − | − | − | − |
| RTM1:RTM1 | − | − | − | − | − | − |
| RTM1:GFP-RTM1 | − | − | + | + | − | − |
| RTM2:RTM2-GFP | − | − | + | − | − | − |
| 35S:GFP | + | + | ndd | + | + | + |
| 35S:GFP-RTM1 | + | + | + | + | + | + |
a
+, Cell type contained GFP when examined by epifluorescence microscopy; −, no fluorescence detected in cell type.
b
DNA construct (see Fig. 2) expressed in transgenic Arabidopsis plants.
c
Phloem-associated cells other than SEs.
d
nd, Not determined. High levels of diffuse GFP in cells and tissues adjacent to SEs prevented determination of whether or not SEs contained GFP.