Table II.

Presequence properties of identified mitochondrial proteins from Arabidopsis

No.a	Protein	Length of Presequence	Ser	Arg/Lys	Glu/Asp	Arg at Cleavage Site?	P of Mitochondria (MITOPROT)b
		no. of amino acids	%
2	ATP synthase, subunit δ′	26	23	19	0	+3	0.985
3	Complex I, 18-kD subunit	11	0	18	0	+4	0.892
4	Unknown mitochondrial protein	89	9	13	3	+2	0.384
5	Gly-rich RNA-binding protein	34	15	9	0	+4	0.743
9	Nucleoside diphosphate kinasec	85	19	11	4	–	0.997
10	Inosine 5-monophosphatedehydrogenase	39	15	10	0	+2	0.855
13	ATP synthase, subunit δ	36	19	17	3	+3	0.881
14	Superoxide dismutase	27	12	26	4	+2	0.960
16	Peroxyredoxin	28	21	18	0	+3	0.985
18	Unknown mitochondrial protein	58	19	16	0	+2	0.989
21	ATP synthase, 24-kD subunit	32	9	12	0	+3	0.900
26	Pyruvate dehydrogenase E1β	37	8	19	5	–	0.910
29	Succinyl-CoA-ligase, β-subunit	26	15	23	0	+2	0.934
32	ATP synthase, subunit β	51	20	18	2	+5	0.997
33	NAD-dependent malatedehydrogenase	22	23	23	0	+2	0.998
35	Succinyl-CoA-ligase, α-subunit	42	26	14	2	+3	0.971
37	Cytochrome b5 reductasec	60	17	8	0	–	0.995
Average		41.4	15.9	16.1	1.35	–	0.904

^a The nos. refer to the nos. given in Figure 3. 

^b The probability for mitochondrial targeting was calculated using MITOPROT (Claros and Vincens, 1996). 

^c These proteins are targeted to the mitochondrial intermembrane space and have a bipartite presequence.