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. 2026 Jun 29;17:1873808. doi: 10.3389/fpls.2026.1873808

Figure 6.

Eight panels labeled A to H show three-dimensional ribbon diagrams of different proteins in blue with binding sites highlighted, each accompanied by close-up insets showing the ligand interaction sites in green with key amino acids labeled and binding affinity values provided. Panels I to N display bar graphs and line plots comparing RNA-seq and qPCR gene expression of six genes (RcUGT13191, RcUGT21426, RcUGT24044, RcUGT5914, RcUGT4379, and RcUGT1732) across three sample types, with statistical differences indicated by letters.

Molecular docking of UGT proteins with tyrosol and expression validation. (A, B) Docking poses of functionally characterized positive controls RrUGT33 and AtUGT85A1 with tyrosol (PubChem CID: 10393). (C–H) Molecular docking conformations of candidate RcUGT genes with tyrosol. (I–N) qPCR expression profiling of candidate RcUGT genes. Legend: (A–H), pTM (predicted TM-score) indicates AlphaFold3 structure prediction confidence. Magnified views (dashed boxes) display hydrogen bonding interactions (yellow dashed lines) between amino acid residues and tyrosol.