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Degradation of bovine MBP by pro- and mature GmMMP2. The proteolytic activity of pro- and mature GmMMP2 purified from E. coli was determined with Tris-HCl, pH 7.5, buffer containing bovine MBP. The reaction mixtures were incubated at 37°C for 0.5, 1, and 4 h. The degrading products were separated with a 4% to 20% (w/v) gradient SDS-PAGE gel and were visualized by Coomassie Brilliant Blue staining. Ctrl, H2O control; Pro, pro-GmMMP2; Mat, mature GmMMP2.
