Figure 7.

Increased protease activity of the suspension-cultured cells upon infection with PsgA. Azocoll-digesting specific activity in the presence and absence of EDTA or a protease inhibitor mixture without EDTA was determined in total soluble extracts from suspension-cultured cells treated with water or infected with PsgA. A, Cells treated with water. B, Cells infected with PsgA in the presence of the protease inhibitor mixture without EDTA. C, Cells infected with PsgA. D, Cells infected with P. syringae in the presence of the proteinase inhibitor mixture and 1 mm EDTA. Two hundred micrograms of total soluble protein extract was used for each protease activity assay.