Neither wounding or callus formation prevents damping. A, Seedlings were entrained for 3 weeks under 12-h light/12-h dark cycles. Leaves were excised as described, and intact leaves and wounded leaves were placed in collars. PHYA:LUC luminescence was assayed in constant darkness. Black triangles, intact leaves. Black squares, wounded leaves. White diamonds, excised leaves. B and C, Calli were produced from excised leaves of plants transformed with the CAB:LUC (B) and PHYA:LUC (C) constructs. The calli were entrained for 3 d under 12-h light/12-h dark cycles, and luciferase activity was assayed in constant darkness. White circles, Callus. Black square, Excised leaves. CAB:LUC values for excised leaves were divided by 50 to bring them within the range of the calli.