Table III.
Accuracy and precision of analysis and purification
| Compound | Accuracy of Analysis (n = 9) | Precision of Purification (n = 10) |
|---|---|---|
| % RSD | ||
| OxIAA-Me | 1.57 | 16.19 |
| IAAla-Me | 0.39 | 14.47 |
| IAAsp-Me2 | 1.27 | 9.79 |
| IAGlu-Me2 | 1.43 | 9.78 |
| IALeu-Me | 2.86 | 5.71 |
Accuracy of the analysis was estimated by three measurements of three Arabidopsis samples pulled together. Precision of purification was estimated by extracting IAA metabolites from 1 g of tissue. The extract was subsequently divided into 10 samples, which were purified and analyzed independently.