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. 2001 Dec;127(4):1854–1862.

Figure 1.

Figure 1

Primer extension analysis transcription start sites of AtPT2 and TPSI1. The transcription start site was mapped to 172 and 91 bp upstream of the translational start site for AtPT2 and TPSI1, respectively. The total RNA isolated from Pi-sufficient (P+) and -deficient (P−) seedlings were used for mapping transcription start site of AtPT2. The poly(A+) RNA (a) and total RNA (b) isolated from Pi-starved roots of tomato were used to map TPSI1 transcriptional start site. DNA sequencing ladders were used to determine the size of the primer extended products.