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Spore formation in gbp mutants
| Strain | No. of viable spores | |
|---|---|---|
| Wild type | 0 | 0 |
| gbpA− | 0 | 1 |
| gbpB− | 28 | 47 |
| gbpA−/gbpB− | 30 | 46 |
Cells were incubated under buffer with cAMP for 36 h, treated with 0.5% NP-40 to kill amoebae, and plated in association with bacteria. The figures refer to the number of colonies obtained in two experiments, each derived from the original 4000 amoebae.
