Table 1.
Intracellular localization of PLD2
| A Organelle | Total gold particles (%) |
|---|---|
| Endoplasmic reticulum | 7.6 ± 2.2 |
| Golgi apparatus | 19.2 ± 0.2 |
| Plasma membrane | 15.7 ± 5.0 |
| Mitochondria | 6.6 ± 0.6 |
| Nucleus | 20.5 ± 9.0 |
| Cytoplasm | 21.9 ± 1.5 |
| Caveolae | 8.5 ± 2.7 |
| B Distribution of PLD2 within organelles | Total gold particles (%) |
|---|---|
| Golgi apparatus | |
| Cisternae | 5.9 |
| ± 4.1 | |
| Rims | 94.1 |
| Plasma membrane | |
| Membrane | 73.8 |
| ± 6.7 | |
| Filopodia | 26.2 |
| C Golgi rim fraction (%) | PLD2 Golgi rim enrichment (fold) | PLD1 Golgi rim enrichment (fold) |
|---|---|---|
| 16.6 ± 0.3 | 80.2 | 1.7 |
A: The intracellular localization of PLD2 immunoreactive gold particles was quantitated from electron micrographs of GH3 cells (N = 58; MATERIALS AND METHODS). The fraction of gold particles on caveolae was quantified independently of the plasma membrane fraction.
B: The numbers of gold particles distributed on Golgi cisternal rims and plasma membrane filopodia were expressed as a percentage of the total immunoreactive gold localized on the respective organelle.
C: The fraction of Golgi rims relative to cisternae was quantitated from electron micrographs of GH3 cells (N = 178) and the relative enrichment of immunoreactive PLD2 gold particles in rims was determined (MATERIALS AND METHODS).