Figure 3.
Flux and sliding in Ncd null mutant embryos. (A) Pole-pole distance as a function of time for wild-type (●) and Ncd null (○) embryos during cycle 12. The bars under the data plots represent the prometaphase isometric state (a) and the metaphase/anaphase A isometric state (b) for wild-type (closed bars) and Ncd null (open bars) embryos. In the Ncd mutants, isometric states are shortened and mitosis proceeds faster. (B) Histogram for the rate of flux during the metaphase/anaphase A isometric state for wild-type (black bars) and Ncd null (gray bars) embryos. The number of counts was normalized to the total number (667 for wild-type and 267 for Ncd null). The average is not significantly different. (C) Histogram for the rate of speckle movement during anaphase B for wild-type (black bars) and Ncd null (gray bars) embryos. The number of counts was normalized to the total number (168 for wild-type and 125 for Ncd null). Both are centered around 0, consistent with MT-MT sliding during anaphase B.