Figure 7.

Effect of kinase inhibitors FL and HD on tau-induced process outgrowth in Sf9 cells. (a) Untreated Sf9 cells (control), showing round shapes) and no cell processes. (b) Sf9 cells transfected with htau23 by baculovirus vector; ∼30% grow extended cell processes up to 100 μm. They also have larger cell bodies (2- to 3-fold). (c) Sf9 cells transfected with htau23 and treated with the kinase inhibitor FL (50 μM). The number of cells with processes increases strongly (∼3-fold). (d) Sf9 cells transfected with htau23 and treated with the kinase inhibitor HD (50 μM). The number of cells with processes drops to ∼10%. (e) Histogram displaying the efficiency of process formation by Sf9 cells in the presence of kinase inhibitors. HD inhibits them strongly, LiCl and H89 promote modestly, and FL promotes strongly.