Table 2.
Comparison of WASP/Scar1 derivatives; bead motility rates vs. Arp2/3 complex stimulation activity
| Bead motility rate (μm/min) | Arp2/3 stimulation | |
|---|---|---|
| WASP | 8.3 ± 0.3 | ++++ |
| WASP-BGPWCA | 5.8 ± 0.3 | +* |
| WASP-GPWCA | 5.3 ± 0.4 | +* |
| WASP-PWCA | 6.1 ± 0.3 | ++++ |
| WASP-WCA | 3.3 ± 0.3 | +++ |
| WASP-CA | 0 | − |
| Scar1 | 5.9 ± 0.4 | ++++ |
| Scar1-PWCA | 6.6 ± 0.4 | +++ |
| Scar1-WCA | 0 | ++ |
| Scar1-CA | 0 | − |
Arp2/3 complex stimulation activities are in the presence of purified WASP family derivatives, Arp2/3 complex, and actin (5% pyrene labeled). Asterisk (
*
) indicates derivatives that are autoinhibited.