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Examination of PI 3-kinase, MAPK, and stress-activated protein kinase pathways activated in response to IGF-I in PTP-1B knockout cells. Serum-starved cells were stimulated with IGF-I for 15 min or left unstimulated. Lysates (25 μg) were analyzed by Western blotting for phospho-AKT or phospho-ERK and for phospho-JNK. Equal loading was assessed by stripping the blots and reprobing with antibodies against the nonphosphorylated forms of AKT, JNK, and ERK.
