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. 2002 Apr;22(7):2136–2146. doi: 10.1128/MCB.22.7.2136-2146.2002

FIG. 3.

FIG. 3.

EBNA-LP and HA95 associate with the catalytic subunit of PKA. (A) Cell lysates from BJAB (B), BJAB-LP (BFLP), IB4, and IB4-LP (IB4FLP) cells were immune precipitated (IP) with anti-Flag antibody. Lysates (5% of starting lysate) and immune precipitates were analyzed by polyacrylamide gel electrophoresis and immune blotted with JF186, HA95, AKAP95, PKA RIIα, and PKAcsα antibodies. (B) BJAB or IB4 cells converted to myc-HA95 expression (BMHA95) or IB4 (IB4MHA) were lysed and immune precipitated with 9E10 anti-myc antibody. Lysates (5% of starting lysate) and immune precipitates were analyzed by polyacrylamide gel electrophoresis and immune blot with PKAcs-specific antibody.