Skip to main content
. 2002 Apr;22(7):2136–2146. doi: 10.1128/MCB.22.7.2136-2146.2002

FIG. 6.

FIG. 6.

HA95 suppresses EBNA-LP coactivation with EBNA-2 of the LMP1 promoter or EBNA-LP coactivation with the EBNA-2 acidic domain fused to the Gal4 DNA binding domain of a promoter with multiple Gal4 DNA binding sites. (A) BJAB cells were cotransfected with the −512/+40 LMP1-luciferase reporter plasmid, pSG-EBNA-2, pSG-EBNA-LP, a control expression vector or a PKAcsα expression vector, and pcDNA3 or pcDNA3myc-HA95 (10 μg of each plasmid). Luciferase activity was measured 48 h later. (B) BJAB cells were cotransfected with pG4tkLuc containing five copies of the Gal4 binding site upstream of a tk promoter-driven luciferase reporter, pSG5-Gal4-EBNA-2AcD, pSG5-EBNA-LP, a control expression vector or a PKAcsα expression vector, and pcDNA3 or pcDNA3myc-HA95 (10 μg of each plasmid). Luciferase activity was measured 48 h later. These results are representative of four independent experiments.