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EBNA-LP coactivation of LMP1 protein expression from the EBV genome in Akata cells is down-regulated by the coexpression of PKAcs or HA95. Akata cells were transfected with pSG5-EBNA-2, pSG5-EBNA-LP, pPKAcs, cPKI, pcDNAmycHA95, or control expression plasmids to balance the transfections (10 μg of each unless otherwise indicated). LMP1 (upper panel) and EBNA-LP (lower panel) expression were assayed by immune blot with anti-LMP1 (S12) and anti-EBNA-LP (JF186) antibody.
