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. 2002 Apr;22(7):2011–2024. doi: 10.1128/MCB.22.7.2011-2024.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — Validation of TAP/DALPC results. (A) S. pombe Cwf5p-myc, Cwf7p-HA, Cwf11p-myc, Cwf12p-HA, Cwf13p-HA, and Cwf17p-myc associate with Cdc5p in vivo. An anti-Cdc5 (left panel) and an anti-myc (right panel) immunoblot of immunoprecipitates (IP) from cwf5-myc, cwf7-HA, cwf11-myc, cwf12-HA, cwf13-HA, and cwf17-myc strains are shown. Immunoprecipitations were performed with preimmune sera (PI), anti-Cdc5p immune sera (I), anti-myc antibodies (myc), or anti-HA antibodies. (B) S. cerevisiae Cwc14p-myc and Cwc15p-myc associate with Cef1p in vivo. An anti-Cef1p (left panel) and an anti-myc (right panel) immunoblot of immunoprecipitates from cwc14-myc and cwc15-myc strains are shown. Immunoprecipitations were performed with preimmune sera (PI), anti-Cef1p immune sera (I), or anti-myc antibodies (myc). (C) S. pombe Lsm8p-TAP and Cdc5p coimmunoprecipitate. Shown is an anti-Cdc5p immunobolot of IgG pulldowns from wild-type (lane 1), arp2-TAP (lane 2), and lsm8-TAP (lane 3) strains.