FIG. 3.

Characterization of S. pombe Cwf11p. (A) The ClustalW 1.6 program (22, 67) was used to align Cwf11-related proteins from H. sapiens (Hs) (O060306), D. melanogaster (Dm) (Q9VGG7), C. elegans (Ce) (Q9U1Q7), A. thaliana (At) (Q9ZVJ8), and S. pombe (Sp) (O94508). Residues found to be identical or similar to those of human CWF11 are highlighted on a solid background or shaded, respectively. (B) cdc5-120 and cwf11::ura4⁺ cells show a reduced restrictive temperature. Wild-type, cdc5-120, cwf11::ura4, and cdc5-120 cwf11::ura4 mutant cells were streaked onto agar medium at 25°C. (C) Tetrads grown at 25°C from the cwf11HA/cwf11⁺ cdc5-120/cdc5⁺ diploid. Only very small colonies grew on plates containing G418, and these were temperature sensitive, indicating that they were double mutants.