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. 2002 Apr;22(7):2011–2024. doi: 10.1128/MCB.22.7.2011-2024.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 5. — Characterization of S. pombe Cwf7p. (A) The ClustalW 1.6 program (22, 67) was used to align Cwf7p-related proteins from H. sapiens (Hs) (O75934), D. melanogaster (Dm) (Q9VAY6), C. elegans (Ce) (Q22417), and S. pombe (Sp) (Q9USV3). Residues found to be identical or similar to those of human CWF7 are highlighted on a solid background or shaded, respectively. (B) cwf7⁺ is required for pre-mRNA splicing. RNAs were prepared from cwf7::ura4⁺ spores after germination, prp2-1 cells grown at 25°C or shifted to 36°C for 2 h, and wild-type cells and were then hybridized to oligonucleotides complementary to the tfIId or his3 mRNAs. PC, precursor; M, mature.

FIG. 5. — Characterization of S. pombe Cwf7p. (A) The ClustalW 1.6 program (22, 67) was used to align Cwf7p-related proteins from H. sapiens (Hs) (O75934), D. melanogaster (Dm) (Q9VAY6), C. elegans (Ce) (Q22417), and S. pombe (Sp) (Q9USV3). Residues found to be identical or similar to those of human CWF7 are highlighted on a solid background or shaded, respectively. (B) cwf7⁺ is required for pre-mRNA splicing. RNAs were prepared from cwf7::ura4⁺ spores after germination, prp2-1 cells grown at 25°C or shifted to 36°C for 2 h, and wild-type cells and were then hybridized to oligonucleotides complementary to the tfIId or his3 mRNAs. PC, precursor; M, mature.