TABLE 2.
Cwc and Cwf proteins identified by DALPC mass spectrometry
| No. | S. cerevisiae Cwc protein (accession no.a) | S. pombe homolog (accession no.a) | Mol wt (kDa) (S. cerevisiae) | Essential?b | Function or motif | No. of hitsc
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cdc5 | Cef1 | Prp19 | Snt309 | Cwc2 | Cwc1 | ||||||
| 1 | Cef1p (Q03654) | Cdc5p (P39964) | 68 | Yes | Required for splicing; Myb repeats | 35 | 11 | 13 | 14 | 16 | 19 |
| 2 | Prp46p/Cwc1p (Q12417) | Prp5p/Cwf1p (AAG01399) | 51 | Yes | WD-40 repeats | 22 | 20 | 13 | 16 | 14 | 20 |
| 3 | Cwc2p (CAA98787) | Cwf2p (P87126) | 41 | Yes | RRMd domains | 11 | 5 | 10 | 1 | 3 | 3 |
| 4 | Syf1p (Q04048) | Cwf3p (Q9P7R9) | 100 | Yes | Required for splicing; TPR repeats | 34 | 18 | 24 | 11 | 1 | 18 |
| 5 | C1f1p (Q12309) | Cwf4p (P87312) | 82 | Yes | Required for splicing; TPR repeats | 28 | 16 | 18 | 9 | 7 | 19 |
| 6 | Ecm2p (P38241) | Cwf5p (O59800) | 41 | Nots | Splicing factor; RRM domain | 15 | 8 | 11 | 5 | 8 | 1 |
| 7 | Prp8p (P33334) | Cwf6p (O10752) | 279 | Yes | Splicing factor, S-phase progression, U5 snRNP protein; polyproline domains | 92 | 38 | 67 | 18 | 7 | 16 |
| 8 | —c | Cwf7p (Q9USV3) | 21 | Yes | Required for splicing | 15 | — | — | — | — | — |
| 9 | Prp19p (P32523) | Cwf8p (O14011) | 51 | Yes | Splicing factor, U box, WD-40 repeats | 24 | 17 | 22 | 18 | 15 | 17 |
| 10 | SmD2p (Q06217) | Cwf9p (O14036) | 13 | Yes | Core Sm protein | 5 | 3 | 3 | 1 | 2 | 3 |
| 11 | Snu114p (P36048) | Cwf10p (SPBC215.12) | 114 | Yes | U5 snRNP component; GTP-binding domain | 38 | 24 | 34 | 12 | 11 | 9 |
| 12 | Isy1p/Ntc30p (P21374) | Cwf12p (O74370) | 28 | No | Auxiliary splicing factor, 2-hybrid interaction with Syf1p, Syf3p, and Cef1p | 9 | 2 | 6 | 3 | 7 | 5 |
| 13 | Snt309p/Ntc25p (Q06091) | — | 21 | Nots | Splicing factor | 9 | 7 | 4 | 13 | 12 | |
| 14 | Ntc20p (P38302) | — | 16 | No | Auxiliary splicing factor | 4 | 3 | 3 | 4 | 1 | |
| 15 | Lea1p (Q08963) | U2Ap (Q9USX8) | 27 | Nots | U2A protein, member of U2 snRNP | 10 | 6 | 5 | 4 | 5 | 4 |
| 16 | Msl1p (P40567) | (O13649) | 13 | No | U2 snRNP component | 1 | 2 | 3 | 2 | 1 | 1 |
| 17 | Brr2p (P32639) | (Q9UT24) | 246 | Yes | Required for splicing, U5 snRNP; DEAD/DEAH RNA helicase | 15 | 17 | 42 | 6 | 1 | 3 |
| 18 | Slu7p (Q02775) | (Q9Y7Y2) | 45 | Yes | 3′ splice choice in 2nd step in splicing | 2 | 1 | 1 | 1 | 2 | N |
| 19 | Syf2p (P53277) | (O59733) | 25 | No | 2-hybrid interaction with Syf1, Syf3, and Cef1p | 7 | 2 | 6 | 1 | 4 | 1 |
| 20 | Prp17p/Cdc40p (P40968) | Prp17p (O43071) | 52 | Nots | Splicing, G2/M progression; WD-40 repeats | 26 | 5 | 7 | 3 | 1 | N |
| 21 | Prp45p (P28004) | Cwf13p (Q09882) | 43 | Yes | SNW domain, polyproline domain, SH2-like domain | 13 | 16 | 13 | 12 | 18 | 18 |
| 22 | Smx3p (P54999) | Smf1p (Q59734) | 9.7 | Yes | Core Sm protein | 2 | 2 | 2 | 2 | 1 | N |
| 23 | SmB1p (P40018) | (Q10163) | 22 | Yes | Core Sm protein | 1 | 5 | 4 | 3 | 6 | 2 |
| 24 | SmD3p (P43321) | Smd3p (Q9UUC6) | 11 | Yes | Core Sm protein | 4 | 2 | 3 | 3 | 3 | N |
| 25 | Cwc14p (P25337) | Cwf14p (O74772) | 18 | No | Hypothetical protein; potential NLSf putative Zn finger | 4 | 2 | 1 | 1 | 1 | 1 |
| 26 | Cwc15p (Q03772) | Cwf15p (O74817) | 20 | No | Hypothetical protein | 6 | 3 | 4 | 3 | 5 | N |
| 27 | Cwc16p (P28320) | Cwf16p (Q9P7C5) | 32 | Yes | Hypothetical protein | 5 | 5 | 8 | N | 6 | 1 |
a
SwissProt or Entrez accession no.
b
Yes, essential gene; No, nonessential gene; Nots, nonessential gene with temperature-sensitive phenotype.
c
Number of unique peptides from each protein that were identified. N, not found in purification.
d
RRM, RNA recognition motif.
e
—, homolog not apparent.
f
NLS, nuclear localization signal.