TABLE 4.
Potential Cwc and Cwf proteins identified by DALPC mass spectrometry
| No. | S. cerevisiae Cwc protein (accession no.a) | S. pombe homolog (accession no.a) | Mol wt (kDa) (S. cerevisiae) | Essential?b | Function or motif | No. of hitsc
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cdc5 | Cef1 | Prp19 | Snt309 | Cwc2 | Cwc1 | ||||||
| 28 | SmD1p (Q02260) | SmD1p (O42661) | 13 | Yes | Core Sm protein | 4 | 1 | 4 | N | 2 | N |
| 29 | Smx2p (P40204) | Smg1 (O74966) | 8 | Yes | Core Sm protein | 4 | 2 | N | 1 | N | N |
| 31 | Ist3p (P40565) | (O94290) | 21 | Yes | U2 snRNP-associated protein | 1 | 1 | 4 | N | 1 | N |
| 32 | Prp9p (P19736) | Sap61p (O59706) | 63 | Yes | U2 snRNP component | 2 | 2 | 5 | N | N | N |
| 33 | Prp11p (Q07350) | SAP62p (Q9P7L8) | 30 | Yes | U2 snRNP component | 3 | 1 | 3 | N | N | N |
| 34 | Prp21p (P32524) | SAP114p (O13900) | 33 | Yes | U2 snRNP component | 1 | 2 | 2 | N | N | N |
| 35 | Hsh155p (P49955) | Prp10p (Q10178) | 110 | Yes | U2 snRNP component | 4 | 1 | 8 | N | N | N |
| 36 | Cus1p (Q02554) | Sap145p (Q9UU13) | 50 | Yes | U2 snRNP component | 2 | N | 4 | 1 | N | N |
| 37 | Rse1p (Q04693) | Prp12p (Q9UTT2) | 154 | Yes | U2 snRNP component | 5 | 1 | 1 | N | N | N |
| 38 | Spp2p (Q02521) | (O43031) | 21 | Yes | Pre-mRNA splicing factor required for final stages of spliceosome maturation | 1 | N | 5 | N | 4 | N |
| 39 | Prp22p (P24384) | Prp22p (O42643) | 130 | Yes | DEAD/DEAH RNA helicase | 16 | 2 | 8 | N | N | N |
| 40 | Cwc21p (Q03375) | Cwf21p (O14161) | 16 | No | Hypothetical RNA binding protein | 4 | 2 | 2 | N | 3 | N |
| 41 | Cwc22p (P53333) | Cwf22p (P78752) | 67 | Yes | Hypothetical protein; H. sapiens (Q9HCG8), D. melanogaster (Q9VJ87), A. thaliana (Q9SAG7), C. elegans (Q17336) | 6 | 2 | 9 | 2 | N | N |
| 42 | Cwc23p (P52868) | Cwf23p (Q9P7C6) | 41 | Yes | Hypothetical protein | N | 2 | 4 | N | N | 2 |
| 43 | Cwc24p (P53769) | Cwf24p (Q9P6R8) | 30 | Yes | Hypothetical protein | 1 | N | 4 | N | 2 | N |
| 44 | Cwc25p (P53854) | Cwf25p (Q9Y805) | 20 | Yes | Hypothetical protein | N | 1 | 3 | N | 3 | N |
| 45 | Cwc26p (P46947) | Cwf26p (O94417) | 31 | No | Hypothetical protein | N | 1 | 2 | N | 2 | N |
| 46 | Cwc27p (Q02770) | Cwf27p (O42941) | 36 | No | Putative peptidyl-prolyl cis-trans isomerase | 6 | 1 | 4 | N | N | N |
a
SwissProt accession no.
b
Yes, essential gene; No, nonessential gene.
c
Number of unique peptides from each protein that were identified. N, not found in purification.