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. 2002 Apr;22(7):2159–2169. doi: 10.1128/MCB.22.7.2159-2169.2002

FIG. 6.

FIG. 6.

Apoptosis induced by NA-AAF in inner cell masses. TUNEL stainings of inner cell masses were visualized by transillumination, and the contrast was artificially enhanced to reveal apoptotic cells (dark spots). As a consequence of the illumination, trophoblast cells are not visible in most of the images. (A to D and G to J) Cells at 16 (A and G), 24 (B and H), 30 (C and I), and 41 (D and J) h after treatment. Note that at 30 h after treatment the inner cell mass of the Rev3−/− blastocyst has partially detached; the remaining part has largely stained black, indicating massive apoptosis. At 41 h most of the inner cell mass of the Rev3−/− blastocyst has detached. (E and K) TUNEL staining of wild-type and Rev3−/− blastocysts, 44 h after treatment with paraoxon alone, revealing similarly low levels of apoptosis for the two genotypes. (F and L) Phase-contrast images of TUNEL staining of a Rev3−/− and a wild-type blastocyst, 44 h after paraoxon treatment, illuminating the reduced size, but normal viability, of the Rev3−/− blastocyst. WT, wild type.