Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Apr;22(7):2025–2036. doi: 10.1128/MCB.22.7.2025-2036.2002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society for Microbiology

PMC Copyright notice

FIG. 1. — Forkhead transcription factors cause upregulation of the p130 pocket protein. (A) MEFs from wild-type (p27^kip1+/+) or p27^kip1 knockout (p27^kip1−/−) mice were infected with either a control retrovirus or an HA-AFX-containing retrovirus. Total cellular lysates were subsequently analyzed for p130, p27^kip1, and HA-AFX expression levels. (B) Purified GST or GST-DBD protein was incubated with a radiolabeled wild-type (p130.DBE) or mutant (p130.DBEmut) probe in the absence or presence of increasing amounts of a nonlabeled oligonucleotide. FL, full length; FP; free probe. (C) A14 or DLD-1 cells were transfected with pGL2-p130intron or pGL2-p130intron.DBEmut, with or without the indicated Forkhead constructs, and luciferase activity was measured. Data are averages from at least three experiments. (D) Two micrograms of RNA was electrophoresed, blotted onto a nylon membrane, and probed for the presence of p130 and GAPDH as a loading control by using radiolabeled probes.

FIG. 1. — Forkhead transcription factors cause upregulation of the p130 pocket protein. (A) MEFs from wild-type (p27^kip1+/+) or p27^kip1 knockout (p27^kip1−/−) mice were infected with either a control retrovirus or an HA-AFX-containing retrovirus. Total cellular lysates were subsequently analyzed for p130, p27^kip1, and HA-AFX expression levels. (B) Purified GST or GST-DBD protein was incubated with a radiolabeled wild-type (p130.DBE) or mutant (p130.DBEmut) probe in the absence or presence of increasing amounts of a nonlabeled oligonucleotide. FL, full length; FP; free probe. (C) A14 or DLD-1 cells were transfected with pGL2-p130intron or pGL2-p130intron.DBEmut, with or without the indicated Forkhead constructs, and luciferase activity was measured. Data are averages from at least three experiments. (D) Two micrograms of RNA was electrophoresed, blotted onto a nylon membrane, and probed for the presence of p130 and GAPDH as a loading control by using radiolabeled probes.