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. 2002 Apr;22(7):2025–2036. doi: 10.1128/MCB.22.7.2025-2036.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 2. — Elevated p130 protein levels are indicative of Forkhead-induced cell cycle exit. (A) Total cellular lysates from NIH 3T3 cells that were either left uninfected (−) or infected either with a control virus (Con) or with an FKHR-L1.A3-expressing retrovirus (L1.A3) were analyzed for the presence of E2F/DP-1/pocket protein complexes. Asterisk, free E2F; FP, free probe. (B) NIH 3T3 cells were infected and analyzed as for panel A. Supershifting antibodies are listed above the gel. Arrows indicate the disappearance of p107/E2F-4 complexes in the third lane from the right, and the appearance of p130/E2F-4 complexes in the second lane from the right, due to Forkhead activity. Asterisk, supershifted cyclin A-containing complexes; a, nonsupershifted E2F/pocket protein complexes; b, free E2F; FP, free probe.