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Inverted repeats stimulate transposition. Transposition reactions were carried out as for Fig. 1B, without ScaI digestion. The target consisted of either a single plasmid or a mixture of F14C and pCDNA1-amp in the indicated mass ratios. For mixtures containing a mixture of targets, the preference for F14C was calculated as the amount of linearized F14C divided by the amount of linearized pCDNA1-amp. All lanes are from the same gel.
