FIG. 5.

Transposition is targeted to hairpins. (A) Transposition was carried out as described in the text. “Precleaved donor” refers to an oligonucleotide donor that corresponds to the RSS after cleavage, i.e., the donor terminates at the site of cleavage (19). The double-stranded control oligonucleotide was FM116/117, and the hairpin oligonucleotide was HY9. Some products of transposition into free donor molecules are visible in the absence of target. The nonspecific target shows no unique bands, while the hairpin target shows several products which correspond to transposition into the hairpin end. (B) The formation of target capture complexes (TCC) was assayed with double-stranded (MM30) and hairpin (HY9) oligonucleotides. HY9 forms substantially more target capture complexes than the MM30 control. The nonspecific shift of target in the absence of donor is indicated (NS). All lanes are from the same gel.