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A cruciform-containing plasmid specifically inhibits hybrid joint formation. (A) Schematic of the hybrid joining assay. A plasmid substrate, pJH299, is incubated with RAG proteins. Hybrid joints are detected by PCR with primers at the indicated sites. (B) The presence of F14C inhibits formation of hybrid joints in this assay, while the presence of pUC8 control has little or no effect.
