FIG.5.
Igf2 RNA FISH in wild-type (A), Dnmt1+/+;BAC (A), Dnmt1−/− (B), Dnmt1chip/− (B), and Dnmt1−/−;BAC (B) cells. Content of images for each cell line is as follows, from left to right: Igf2 expression (green), nuclear 4′,6′-diamidino-2-phenylindole (DAPI) staining (blue), and merged image of Igf2 and DAPI. The graph to the right indicates the monoallelic versus biallelic Igf2 expression observed in each cell line. (C) Igf2 and H19 RNA FISH of wild-type and Dnmt1−/−;BAC cells. Images, from right to left, are Igf2 expression (green), H19 expression (red), a merged imaged of Igf2 and H19 expression, and a merged image of Igf2 and H19 expression with nuclear DAPI staining.