Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Spag5−/− mice lack Spag5 mRNA. mRNA was isolated from the testes of Spag5−/− mice (lane 1) and Spag5+/− heterozygotic mice (lane 2), and 5 μg of mRNA from each was analyzed by Northern blotting hybridization with a 5′ Spag5 cDNA fragment as probe. After autoradiography, filters were stripped of the probe and rehybridized to a GAPD (glyceraldehyde-3-phosphate dehydrogenase) cDNA probe to confirm the quantity and quality of mRNA for both preparations (lanes 3 and 4).
