FIG. 7.

Cav-2-null mice have normal body weights, adipose tissue morphology, and serum metabolites. (A) Routine histology (H&E) was performed on several different adipose tissue regions (perigonadal fat pads are shown) of 1-year-old wild-type and Cav-2-null mice, an age at which the adipose tissue of Cav-1-null mice has been shown to display heterogeneously sized adipocytes, marked interstitial fibrosis, and hypercellularity. No differences between wild type and Cav-2 knockouts (KO) are apparent. (B) The entire left-side mammary gland 4 of wild-type, Cav-2-knockout, and Cav-1-knockout females was dissected and subjected to whole-mount preparation. Carmine dye staining of the mammary tissue allowed visualization of the ductal architecture and density. (C) Fasting blood samples were collected at 7:00 a.m. (12 h after removal of food) and postprandial blood was collected at 12:00 a.m. (after 3 h of feeding in the dark) from 3-month-old wild-type, Cav-2-knockout (KO), and Cav-1-knockout cohorts. Glucose, cholesterol, triglyceride, and free fatty acid levels in plasma were measured colorimetrically.