. 2002 Apr;22(7):2047–2056. doi: 10.1128/MCB.22.7.2047-2056.2002

TABLE 1.

Two-hybrid assay between Ime1 and Ime2

Fusion protein^a	β-Galactosidase (U) (relative level of expression)
Fusion protein^a	SD	SPM3^b	SPM6^b
Gal4(bd)-Ime2(1-645) + Gal4(ad)	4.3 (1.0)	9.4 (1.0)	21.5 (1.0)
Gal4(bd)-Ime2(1-645) + Ime1 (1-360)	18.5 (4.3)	46.5 (4.9)	352.9 (16.4)
Gal4(bd)-Ime2(1-645) + Gal4(ad)-Ime1(270-360)	28.8 (6.7)	109.0 (11.6)	169.0 (7.9)

Strain Y153 (17) was used. Plasmids used were YEp1916 for Gal4(bd)-Ime2(1-645), YEp53 for Ime1, pGAD2F (9) for Gal4(ad), and YEp1406 for Gal4(ad)-Ime1 (270-360).

SPM3 and SPM6, 3 and 6 h of incubation, respectively, in SPM.