FIG. 5.
A combined absence of telomerase and Yku does not lead to immediate growth arrest in cells with elongated telomeric repeat tracts. Strain SGY53 (yku70Δ tlc1Δ rif1Δ pKu70-30ts) and the same strain carrying in addition a plasmid-borne copy of TLC1(pAZ1) were grown at 23°C in liquid medium. To assess viability of these strains, 10-fold serial dilutions of the cells were spotted on SC-Trp plates and incubated at 23 or 37°C. tlc1Δ(a) and -(b), two independent clones of the strain without pAZ1.