TABLE 1.
Telomere lengths and growth phenotypes of Yku mutants
| Strain genotype | Telomere lengtha and growthb under conditions of:
|
|||||
|---|---|---|---|---|---|---|
| Pregrowth at 23°C, plate growth at 23°C
|
Pregrowth for 2.5 generations at 37°C, plate growth at 37°C
|
Pregrowth for 6 generations at 37°C, plate growth at 37°C
|
||||
| Telomere length (bp) | Growth | Telomere length (bp) | Growth | Telomere length (bp) | Growth | |
| yku70Δ | 130 (55-230) | + | 95 (40-180) | − | N.A.d | N.A. |
| yku70Δ rif1Δ | 250 (100-380) | + | 205 (80-330) | +/− | 155 (75-280) | − |
| yku70-30ts | 265 (160-395) | + | 220 (110-360) | +/− | 180 (76-330) | − |
| yku70-30ts rif1Δ | 620 (385-880) | + | 565 (360-875) | + | 465 (280-720) | + |
| YKU70 | 275 (185-370) | + | N.D.c | + | 265 (145-360) | + |
a
Telomere length after the pregrowth period was calculated from Southern analysis (Fig. 3B) as described in Materials and Methods. The means and ranges are indicated.
b
The growth phenotype was evaluated from Fig 3A. +, formation of colonies similar to wt; +/−, formation of microcolonies; −, microcolonies not detectable in spots of the diluted culture.
c
N.D., not determined.
d
N.A., not applicable due to cell mortality.