TABLE 1.
Interaction of RACK1 with IGF-IR in yeast is specific when compared to IR
| Baita | Preyb | Growthc | Colord |
|---|---|---|---|
| IGF-IR | Empty | No | − |
| IGF-IR | RACK1 | Yes | ++ |
| IR | Empty | No | − |
| IR | RACK1 | No | − |
| IR | Stat5bCT | Yes | ++++ |
The yeast two-hybrid interaction trap was used to examine the interaction of the tested proteins as described in Materials and Methods. Kinase active cytoplasmic domains of IGF-IR and IR were used as bait.
Library plasmids isolated originally from two-hybrid screens were used as prey. pJG4-5 empty plasmid was used as control.
Inducible colony growth after 48 h on galactose Leu− plates was compared to glucose Leu− plates.
Inducible colony color on galactose-X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) plates was compared to that on glucose-X-Gal plates within 24 h. The number of “+” symbols indicates the relative intensity of the blue color, and the minus sign indicates white color.