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. 2002 Apr;22(8):2830–2841. doi: 10.1128/MCB.22.8.2830-2841.2002

FIG. 3.

FIG. 3.

Immunohistochemical analysis of Notch4IC expression in the CAM. Q2bn packaging cells transfected with the vector control (Q2bn-CK) or Notch4IC (Q2bn-Notch4IC) were placed onto nylon meshes on the CAM surface. Treated CAMs were harvested on day 12, and sections were prepared. (A to D) CAM sections proximal to mesh. Shown is H&E staining of Q2bn-CK-treated (A) and Q2bn-Notch4IC- treated (B) CAMs and anti-vWF staining of Q2bn-CK-treated (C) and Q2bn-Notch4IC-treated (D) CAMs. (E to H) CAM sections distant from mesh. Shown is anti-HA staining of Q2bn-CK-treated (E) and Q2bn-Notch4IC-treated (F) CAMs and anti-vWF staining of Q2bn-CK-treated (G) and Q2bn-Notch4IC-treated (H) CAMs. Original magnifications: ×40 (A to D, F, and H) and ×63 (E and G).