Figure 4 – figure supplement 1.
a. Quantification of CD44 expression by donor-origin naïve CD8 T cells in Figure 4b. b. Quantification of CD44 expression by recovered donor-origin CD8 T cells in Figure 4c. c. As in Figure 4c, for the cells recovered from pooled brachial, axial and inguinal lymph nodes. d.-j. Bone marrow chimeras generated from Bcl11fl/+Cd4-creTg and Cre-expressing donors analysed at the indicated timepoints post transfer. d. CD44 expression by recovered naïve donor-origin cells at 4 weeks. e. Quantification (left) and representative histograms (right) of Ly6C expression by donor-origin naïve CD8 T cells at 4 weeks. f.-g. As for Figure 4e, at 5 weeks post reconstitution. h. and i., as for d. and e., at 5 weeks post reconstitution. j. Representative flow cytometry plots (left) and quantification (right) of CD73 expression by mature single positive thymocytes 4 weeks post reconstitution. Significance tested using one-way ANOVA with Šidák’s correction for multiple comparisons (g, j) or Student’s two-tailed t test (a-f, h, i).