Figure 3. The influence of the nucleotide context of the cleavage site on the efficiency of Nsp1-mediated RNA cleavage.

(A-B) Upper panels - schematic representation of 34nt.-long 5’UTRs comprising CAA repeats interrupted by G (red) at indicated positions and containing nucleotide variations at the cleavage site. Lower panels - Nsp1-mediated cleavage of mRNAs with UTRs shown on the upper panels in the presence of the indicated translation components, assayed by primer extension. Cleavage sites are shown on the right and marked on the 5’ UTRs in the upper panels (red arrows).