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[Preprint]. 2026 Jun 22:2026.06.18.733267. [Version 1] doi: 10.64898/2026.06.18.733267

Figure 6. Nsp1-mediated cleavage of SARS-CoV-2 mRNAs.

Figure 6.

(A) Upper panel - secondary structure models of the wt and mutant SARS-CoV-2 SL1. G nucleotides are in red, introduced mutations are in green. Lower panel - Nsp1-mediated cleavage of wt and mutant SARS-CoV-2 mRNAs shown in the upper panel in the presence of the indicated translation components, assayed by primer extension. Cleavage sites are indicated on the right and marked on the SL1 models shown in the upper panel (red arrows). Lanes C, T, A, and G show the wt sequence derived using the same primer as for primer extension. (B) Left panel – mutations introduced into SL1 of the 5’-terminally truncated (D7 nt.) SARS-CoV-2 5’UTR. Right panel - Nsp1-mediated cleavage of wt and mutant SARS-CoV-2 mRNAs (shown in the left panel) in the presence of the indicated translation components, assayed by primer extension. Cleavage sites are indicated on the right (red arrows). (C) Nsp1-mediated cleavage of full-length SARS-CoV-2 mRNAs containing mutations identical to those that were introduced into the 5’-terminally truncated SARS-CoV-2 5’UTR (B, left panel). mRNAs were incubated with Nsp1 and the indicated translation components and assayed by primer extension. The cleavage site is indicated on the right. Lanes C, T, A, and G show the wt sequence derived using the same primer as for primer extension.