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[Preprint]. 2026 Jun 22:2026.06.18.733267. [Version 1] doi: 10.64898/2026.06.18.733267

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Figure 7. — (A) The influence of Nsp1 on 48S complex formation on mRNAs containing genomic and ORF8 subgenomic SARS-CoV-2 5’UTRs in the presence of the indicated translation components, assayed by toe-printing. The positions of 48S complexes are indicated. (B) The influence of Nsp1 on translation of mRNA containing the genomic SARS-CoV-2 5’UTR in RRL supplemented with the indicated amounts of Nsp1, assayed in the presence of ³⁵S-Methionine. (C) The influence of Nsp1 on competition between β-globin mRNA and mRNA containing the genomic SARS-CoV-2 5’UTR during translation in RRL supplemented with the indicated amounts of Nsp1, assayed in the presence of ³⁵S-Methionine. (B, C) Translation products resolved by SDS-PAGE are indicated on the right. (D, E) The integrity of (D) β-globin mRNA and (E) mRNA containing the genomic SARS-CoV-2 5’UTR after translation of their mixture in RRL supplemented by the indicated amounts of Nsp1. After translation, mRNAs were phenol extracted, ethanol precipitated and assayed by primer extension. Cleavage sites (in D) are shown on the right (red).