FIG. 2.

SF/HGF overcomes serum starvation growth inhibition in U373MG cells. (A and B) Fluorescence-activated cell sorter analysis of U373MG cells grown in 0.1% FBS showed an increase in the G₁/G₀ fraction compared to cells in 10% FBS. Addition of 100 ng of SF/HGF per ml to U373MG cells for 24 h in low serum blocked serum starvation growth inhibition, reducing the G₁/G₀ fraction by 25.3% (n = 3 experiments, SE = 5.1%) compared to that of untreated controls. SF/HGF increased tritiated thymidine incorporation (C) in U373MG cells grown in low serum by 37.4% (n = 6 per group, SE = 2.7%, P < 0.001, t test) and MTT conversion (D) to formazen by 43.9% (n = 8 per group, SE = 0.2%, P < 0.001, t test)