FIG. 6.

p27 is not required for growth arrest or SF/HGF-mediated mitogenic response. (A) Fluorescence-activated cell sorter analyses of U373MG cells transiently transfected with expression plasmids encoding wild-type p27 or a proteasome-resistant p27 mutant T187A demonstrate no effect of p27 on cells in the log growth phase in 10% FBS. Transfection efficiency as judged by cotransfection with a plasmid encoding GFP was greater than 80%. (B) Overexpression of p27 or p27T187A did not block SF/HGF-mediated mitogenesis in U373MG cells. (C) Western blot analysis of transfected cells demonstrates increased p27 protein following transient transfection. SF/HGF was able to downregulate exogenous wild-type p27, but not the degradation-resistant p27T187A mutant.