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c-Myc is a delayed-early-response gene for SF/HGF. (A) U373MG cells were grown in 0.1% FBS for 24 h, pretreated with 10 μM cycloheximide, stimulated with 100 ng of SF/HGF per ml, and subjected to analysis by Northern blotting with full-length c-myc and GAPDH cDNA probes. (B and C) Western blot analysis for c-Myc protein levels following stimulation of U373MG cells with 100 ng of SF/HGF per ml arrested by contact inhibition in 10% FBS (B) or by serum starvation in 0.1% FBS (C).
