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Generation of AY and p53S23A MEFs by Hprt-deficient blastocyst complementation. Southern blot analysis of genomic DNA from passage 3 MEFs derived from embryos generated by injection of AY or p53S23A ES cells into Hprt-deficient blastocysts. Genomic DNA was digested with EcoRI and hybridized with probe A (see Fig. 1). Lane 1, wild-type MEFs; lane 2, AY MEFs; lane 3, p53S23A MEFs.
