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. 2002 Apr;22(8):2607–2619. doi: 10.1128/MCB.22.8.2607-2619.2002

FIG. 3.

FIG. 3.

(A) Northern blot analysis of total RNA from thioglycolate-elicited peritoneal macrophages of PPARγ-MXCre− and PPARγ-MXCre+ mice treated with saline or pIpC, as indicated. Macrophages were treated with the indicated ligands [15 μM troglitazone (T), 2.5 μg of 22-HC/ml, 100 nM dexamethasone (D), and DMSO vehicle (C)] and total macrophage RNA (10 μg) was denatured and electrophoresed in formaldehyde-containing 1% agarose gels, blotted to nylon membranes, and probed with the indicated cDNA probes for PPARγ and β-actin. (B) RNase protection assay. Total RNA from thioglycolate-elicited peritoneal macrophages of pIpC-treated PPARγ-MXCre− (lane 1), saline-treated PPARγ-MXCre+ (lane 2), and pIpC-treated PPARγ-MXCre+ (lane 3) mice were hybridized with riboprobes for β-actin and PPARγ (as described in Materials and Methods) and subjected to digestion with RNase H. The products were then separated on a 5.0% polyacrylamide gel. Positions of the protected mRNA fragments for PPARγ and β-actin, as well as the expected size of the PPARγ truncated mRNA, are indicated.