FIG. 5.

Northern blot analysis of total RNA from thioglycolate-elicited peritoneal macrophages of PPARγ-MXCre⁻ and PPARγ-MXCre⁺ mice treated with saline or pIpC, as indicated. Macrophages were treated with the indicated ligands [15 μM troglitazone (T), 2.5 μg of 22-HC (HC)/ml, 100 nM dexamethasone (D), and DMSO vehicle (C)], and total macrophage RNA (10 μg) was denatured and electrophoresed in formaldehyde-containing 1% agarose gels, blotted onto nylon membranes, and probed with the indicated cDNA probes for CD36, LPL, and β-actin. The intensities of hybridizing mRNA bands were quantitated and normalized to β-actin mRNA. Numbers represent signal intensities relative to pIpC-treated Cre⁻ macrophages (lane 1).