FIG. 7.

Northern blot analysis of total RNA from thioglycolate-elicited peritoneal macrophages of PPARγ-MXCre⁻ and PPARγ-MXCre⁺ mice treated with pIpC, as indicated. Macrophages were treated with the indicated ligands [15 μM troglitazone (T), 5 μM rosiglitazone (R), 10 μM pioglitazone (P), 13 μM ciglitazone (Ci), and DMSO vehicle (C)], and the total macrophage RNA (10 μg) was denatured and electrophoresed in formaldehyde-containing 1% agarose gels, blotted onto nylon membranes, and probed with the indicated cDNA probes for ABCA1, CD36, and β-actin. The intensities of hybridizing mRNA bands were quantitated and normalized to β-actin mRNA. The numbers represent signal intensities relative to pIpC-treated Cre⁻ macrophages (lane 1).