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. 2002 Apr;22(8):2586–2597. doi: 10.1128/MCB.22.8.2586-2597.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 4. — 3TP dual-luciferase reporter assay in K562 cells transiently expressing ALK4-Flag, ALK4-2-Flag, CR-1, and/or Nodal. (A) Human K562 erythroleukemia cells were transfected with 0.5 μg of different plasmid DNAs together with Renilla luciferase control reporter vector (pRL-TK). Twenty-four hours after transfection, the cells were lysed and luciferase activity was measured using the Dual-Luciferase Reporter Assay system (Promega). M1/M2, plasmid DNA/control reporter vector ratio; RLU, relative light units. (B) Coimmunoprecipitation of dominant-negative ALK4-2-Flag with CR-1 in COS1 cells. COS1 cells transiently transfected with ALK4-2-Flag and/or CR-1 cDNAs were immunoprecipitated (IP) with an anti-Flag mouse monoclonal antibody and analyzed by Western blot (WB) using an anti-CR-1 rabbit polyclonal antibody or an anti-Flag mouse monoclonal antibody. MW, molecular weight in thousands; C, control.