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RT-PCR and Western blot analysis for Nodal in EpH-4 WT and Eph-4 N cells. (A) RT-PCR with specific Nodal primers (313 bp) in EpH-4 WT and EpH-4 N cells. (B) Western blot analysis using a rabbit polyclonal anti-Nodal antibody in the culture medium of EpH-4 WT and EpH-4 N cells. MW, molecular weight in thousands.
